Error rate in transcription

Value 1E-05 mutation/nucleotide Range: 5e-6 - 5e-5 mutation/nucleotide
Organism Bacteria Escherichia coli
Reference Ninio J. Connections between translation, transcription and replication error-rates. Biochimie. 1991 Dec73(12):1517-23. p.1518 left column 2nd paragraphPubMed ID1805967
Primary Source (13) Blank A, Gallant JA, Burgess RR, Loeb LA. An RNA polymerase mutant with reduced accuracy of chain elongation. Biochemistry. 1986 Oct 7 25(20):5920-8. (14) Rosenberger RF, Hilton J. The frequency of transcriptional and translational errors at nonsense codons in the lacZ gene of Escherichia coli. Mol Gen Genet. 1983 191(2):207-12.
Method Primary source (13): "The novel isolation procedure consisted of simultaneous selection for rifampicin resistance and screening for increased leakiness of an early, strongly polar nonsense mutation of lacZ, one of a special class of mutations whose leakiness reflects mainly transcriptional rather than translational errors." Primary source (14): In vivo methods
Comments P.1518 left column 2nd paragraph: "The transcription error-rate is about 0.5 to 1.0×10^-5 per nucleotide, according to in vitro estimates [Primary source (13)] but may reach 5×10^-5 at some loci in vivo [Primary source (14)]. The reasoning will be in terms of a standard transcription error-rate of 10^-5." See BNID 103453 for misincorporation rate of 10^-4 in transcription. Value of 10^-5 also given by Ortego et al 2007 PMID 17985929.
Entered by Uri M
ID 105212