Number of proteins/mRNA

Value ~540 proteins/mRNA Range: 300 to 600 proteins/mRNA
Organism Bacteria Escherichia coli
Reference Lu P, Vogel C, Wang R, Yao X, Marcotte EM. Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation. Nat Biotechnol. 2007 Jan25(1):117-24. abstract, p.120 caption to figure 4b,e, p.121 right column 3rd paragraph and p.122 left column 4th paragraphPubMed ID17187058
Method Abstract: "[Researchers] report a method for large-scale absolute protein expression measurements (APEX) and apply it to estimate the relative contributions of transcriptional- and translational-level gene regulation in the yeast and Escherichia coli proteomes. APEX relies upon correcting each protein's mass spectrometry sampling depth (observed peptide count) by learned probabilities for identifying the peptides."
Comments P.121 right column 3rd paragraph: "The E. coli proteome revealed a similar trend—APEX-based protein abundances correlated with average absolute mRNA abundances from at least two of three experiments [refs 30,31,32] (Rs = 0.69, R2 = 0.47, Fig. 4d). Again, the relationship is well described by a linear relationship [protein] = 540 × [mRNA], with ∼300–600 proteins/mRNA (Fig. 4e), depending upon details of normalization (Supplementary Notes and Supplementary Data 3). As with yeast, the logarithm of the ratio of proteins per mRNA is well fit (R2 = 0.94) by a normal distribution (Fig. 4f)." Note-Arkin et al 1998 give value of 10 proteins/mRNA, BNID 100196.
Entered by Uri M
ID 104186