||Budding yeast Saccharomyces cerevisiae
||Cha JY, Park JC, Jeon BS, Lee YC, Cho YS. Optimal fermentation conditions for enhanced glutathione production by Saccharomyces cerevisiae FF-8. J Microbiol. 2004 Mar42(1):51-5. p.52 left column bottom paragraph & p.54 left column 3rd paragraphPubMed ID15357293
||P.51 right column top paragraph: "Saccharomyces cerevisiae FF-8, a glutathione producing yeast strain, was established in [investigators'] laboratory (Park et al., 2003). FF-8 was aerobically grown in 500 ml flask containing 100 ml of YM medium consisting of 1.0% glucose, 0.5% peptone, 0.3% yeast extract, and 0.3% malt extract, at pH 6.0 for 24 h at 30˚C." P.51 right column bottom paragraph: "The harvested yeast cells were suspended in 0.2 M phosphate buffer (pH 7.2) and disrupted by sonication. The disrupted cells were then removed by centrifugation, and glutathione concentration in the supernatant was measured using published methods (Cohn et al., 1966), by measuring the absorbance of reaction
solutions at 412 nm using a spectrophotometer (UV mini 1240, Shimadzu, Japan)."
||P.52 left column bottom paragraph: "Yeast strains such as S. cerevisiae and Candida utilis have been reported to produce glutathione (Wei, 2003 Alfafara et al., 1992). [Investigators] have previously reported that S. cerevisiae FF-8, a glutathione producing strain, was isolated from Korean Traditional Rice Wine. Glutathione production by S. cerevisiae FF-8 under optimal culture conditions in YM medium was 90 mg/l." P.54 left column 3rd paragraph: "The glutathione concentration produced by S. cerevisiae FF-8 using this medium significantly increased by 2.27-fold [to 204 mg/l] compared to the 90 mg/l achieved in YM medium."